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Utah Public Health Laboratory

Burkholderia mallei and Burkholderia pseudomallei (Glanders & Melioidiosis)

Fee:Please refer to the fee schedule

Ordering Recommendation

All clients – Contact Utah Public Health Laboratory prior to submitting specimens

Performed

Sunday - Saturday as required.

Methodology

LRN Protocols

Reported

<1-7 days

Specimen Required

  • Collect:Culture isolate, blood, serum, urine, abscesses, tissue aspirates, body fluids (throat, nasal, skin or sputum for intentional release exposures.
  • Specimen Preparation:
    • Blood – Collect blood specimens before antibiotics are administered, when possible. Collect appropriate volume and number of sets per laboratory protocol.
    • Urine – Collect a midstream clean-catch specimen or a catheterization specimen.
    • Abscesses, tissue aspirates, fluids – Collect tissues and fluids rather than swabs, when possible.
    • Special situations – Throat, nasal, skin or sputum specimens may be helpful in screening exposed individuals if a release of B. mallei or B. pseudomallei has been confirmed
  • Storage/Transport Conditions:For transport time >2h and <24h, refrigerate at 2 to 8°C. Culture isolates may be transported at room temperature.
  • Unacceptable Conditions: Calcium alginate or cotton swabs, swabs with wooden shaft, or dry swabs.
  • Remarks:
  • Stability: Transport directly to laboratory at room temperature. For transport time >2 h and < 24 h, refrigerate at 2 to 8°C.

Results

Detected or Not Detected

Recovered or Not Recovered

Interpretive Data

Burholderia species DNA detected or not detected by PCR.

Burkholderia species recovered or not recovered from culture.

Note

It is mandatory that UPHL be contacted prior to submitting samples for testing.

Cross References

American Society of Microbiology Sentinel Level Clinical Laboratory Guidelineshttp://www.asm.org/index.php/guidelines/sentinel-guidelines