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Utah Public Health Laboratory

Coxiella burnetti (Q fever)

Fee: Please refer to fee schedule

Ordering Recommendation

All clients – Contact Utah Public Health Laboratory prior to submitting specimens.

Performed

Sunday - Saturday as required

Methodology

LRN Protocols

Reported

<1-2 days

Specimen Required

  • Collect:Environmental samples, blood, serum, nasopharyngeal swab, bronchial/tracheal washing or lesion exudate
  • Specimen Preparation:
    • Blood: Collect blood in EDTA (lavender) or sodium citrate (blue) Vacutainer tubes and maintain at 4°C for storage and shipping for PCR or special cultures. If possible, collect specimens prior to antimicrobial therapy. o
    • Serum: Collect serum (red-top or serum separator tube, tiger-top tube) as soon as possible after onset of symptoms (acute phase) and with a follow-up specimen (convalescent phase) at ≥ 14 days for serological testing.
    • Tissue, body fluids, nasopharyngeal swabs, tracheal/bronchial washings, lesion exudates: Specimens can be kept at 2-8°C if transported within 24 hours. Store frozen at -70°C or on dry ice.
  • Storage/Transport Conditions:For transport keep specimens refrigerated at 2 to 8°C.
  • Unacceptable Conditions:Calcium alginate or cotton swabs, swabs with wooden shaft, or dry swabs.
  • Remarks:
  • Stability:For transport keep specimens refrigerated at 2 to 8°C.

Results

Detected or Not Detected

Interpretive Data

Coxiella burnetti DNA detected or not detected by PCR

Note

It is mandatory that UPHL be contacted prior to submitting samples for testing.

Cross References

American Society of Microbiology Sentinel Level Clinical Laboratory Guidelineshttp://www.asm.org/index.php/guidelines/sentinel-guidelines