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Utah Public Health Laboratory

Francisella tularensis (Tularemia)

Fee:Please refer to fee schedule

Ordering Recommendation

All clients – Contact Utah Public Health Laboratory prior to submitting specimens.

Performed

Sunday - Saturday as required.

Methodology

LRN Protocols

Reported

<1-7 days

Specimen Required

  • Collect:Culture isolate, environmental samples, blood cultures, biopsied tissue, ulcer or lesion scraping or aspirate, lesion swabs, sputum, bronchial/tracheal aspirate.
  • Specimen Preparation:
    • Blood Culture (Septicemic) – Collect appropriate blood volume and number of sets per established laboratory protocols. Standard blood culturing system (10-20 ml/bottle). Transport directly to Sentinel Laboratory at room temperature. Hold at room temperature until placed onto the blood culture instrument or incubator. Do not refrigerate. Follow established laboratory protocol for processing blood cultures.
    • Biopsied tissue or scraping/aspirate of ulcer or lesion – A swab of the ulcer is an acceptable alternative. Submit tissue, scraping, or aspirate in a sterile container. For small tissue samples, add several drops of sterile normal saline to keep the tissue moist.
    • Swabs: Obtain a firm sample of the advancing margin of the lesion. If using a swab transport carrier, the swab should be reinserted into the transport package and the swab fabric moistened with the transport medium inside the packet.
    • Lower respiratory tract (pneumonic) sputum or aspirate – Transport specimen (>1 ml) in a sterile, screw-capped container.
  • Storage/Transport Conditions:For transport time >2h and <24h, refrigerate at 2 to 8°C. Culture isolates may be transported at room temperature.
  • Unacceptable Conditions:Calcium alginate or cotton swabs, swabs with wooden shaft, or dry swabs.
  • Remarks:
  • Stability: Transport directly to laboratory at room temperature. For transport time >2 h and < 24 h, refrigerate at 2 to 8°C.

Results

Detected or Not Detected

Recovered or Not Recovered

Interpretive Data

Francisella tularensis DNA detected or not detected by PCR.

Francisella tularensis recovered or not recovered from culture.

Note

It is mandatory that UPHL be contacted prior to submitting samples for testing

Cross References

American Society of Microbiology Sentinel Level Clinical Laboratory Guidelineshttp://www.asm.org/index.php/guidelines/sentinel-guidelines