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Utah Public Health Laboratory

Orthopox viruses

Fee:Please refer to fee schedule

Ordering Recommendation

All clients – Contact Utah Public Health Laboratory prior to submitting specimens.

Performed

Sunday - Saturday as required.

Methodology

LRN Protocols

Reported

<1-2 days

Specimen Required

  • Collect: Biopsy, scabs, vesicular fluid, vesicular swabs, and vesicular tissue.
  • Specimen Preparation:o
    • Biopsy – Aseptically place two to four portions of tissue into a sterile, leakproof, freezable container.
    • Scabs – Aseptically place scrapings/material into a sterile, leak-proof, freezable container.
    • Vesicular fluid – Collect fluid from separate lesions onto separate sterile swabs. Be sure to include cellular materials from the base of each respective vesicle.
  • Storage/Transport Conditions:If transport time will be ≤6 hours, transport at 4°C. Store specimens at -20°C to -70°C.
  • Unacceptable Conditions: Calcium alginate or cotton swabs, and swabs with wooden shaft.
  • Remarks:
  • Stability: For transport keep specimens refrigerated at 2 to 8°C. If transport time will be ≤6 hours, transport at 4°C. Store specimens at -20°C to -70°C.

Results

Detected or Not Detected

Interpretive Data

Orthopox virus DNA detected or not detected by PCR.

Note

It is mandatory that UPHL be contacted prior to submitting samples for testing.

Cross References

American Society of Microbiology Sentinel Level Clinical Laboratory Guidelineshttp://www.asm.org/index.php/guidelines/sentinel-guidelines

Herpes Panel by PCR